Cryopreservation and resuscitation method for keeping sperm insemination performance of large yellow croaker and application of cryopreservation and resuscitation method

• Main Authors: YAN GUIYANG, HAN KUNHUANG, ZHOU FENGFANG, LIN YAN, LIU ZHENG, LIN PEIHUA, GUO TUANYU, ZHANG YI, HUANG WEIQING, RUAN SHAOJIANG
• Format: Patent
• Language: Chinese; English
• Published: 22.03.2022
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; NEW BREEDS OF ANIMALS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; TRAPPING; VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS

The invention provides a cryopreservation and resuscitation method for keeping sperm insemination performance of large yellow croaker and application, and the method specifically comprises the following steps: selecting male parent fish of large yellow croaker with good gonad development, smooth body surface and strong vitality, anesthetizing the male parent fish with eugenol, and injecting an oxytocic from the base of pectoral fins; within 32-34h after injection, collecting seminal fluid of the male parent fish, and testing sperm motility; preparing the large yellow croaker semen with the sperm motility not lower than 90% and the cryopreservation liquid into a mixed solution, and filling the mixed solution into a cryopreservation tube; the cryopreservation tube is placed in an environment of-20 DEG C to-50 DEG C to be pre-cooled for 10-15 min, and then the cryopreservation tube is placed in liquid nitrogen of-180 DEG C to be stored for not more than 60 days; and taking out the cryopreservation tube from the