High performance liquid chromatography detection method for gingerol component content

• Main Authors: WENG CHENGWU, HUANG HUOSHUI, RONG JIEFENG, WANG QINGXIN
• Format: Patent
• Language: Chinese; English
• Published: 18.03.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a high performance liquid detection method for the content of gingerol components. According to the technical scheme, firstly, response characteristics of gingerol components in HPLC detection are analyzed, and a specific detection method is designed on the basis. Specifically, the method comprises the following steps: respectively preparing a to-be-detected sample and a reference substance solution by using a methanol solution, carrying out column chromatography by using amylose-tri (3-chloro-5-methyl phenyl carbamate) as a filler on the basis, and adopting an ethanol-phosphate solution and acetonitrile as mobile phases under the conditions that the column temperature is 34-38 DEG C, the detection wavelength is 330-350 nm, the flow rate is 0.3-0.6 mL/min and the sample size is 30 [mu] L; linear gradient elution is adopted, and the content is calculated according to the peak area by adopting an internal standard method after detection. The method is simple and convenient to operate, the