Method for detecting biomolecules based on G4 nucleic acid aptamer

• Main Authors: PENG JIASHENG, HE CHANG, LI ZHENG, FANG JUN, FENG GUANGFU, LIAO SIJIE, ZHANG HUAIZU, JIANG HONGMEI, FEI YANQUAN
• Format: Patent
• Language: Chinese; English
• Published: 18.03.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING QUANTITY OF HEAT; MEASURING TEMPERATURE; PHYSICS; TESTING; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR

The invention relates to the technical field of biosensors, and discloses a method for detecting biomolecules based on a G4 nucleic acid aptamer. The method comprises the following steps: (1) in a reaction solvent I, carrying out mixed reaction on G4 nucleic acid aptamers of biomolecules and potassium ions to obtain a reaction solution I; (2) mixing the reaction solution I, chlorhematin and a to-be-detected substance containing the biomolecules I to obtain a reaction solution II; (3) mixing the reaction solution II, hydrogen peroxide and 3, 3 ', 5, 5'-tetramethyl benzidine to obtain a reaction solution III, observing the color change of the reaction solution III and detecting the light absorption value, and (4) irradiating the reaction solution III with laser and detecting the temperature change after the laser is shut down. 本发明涉及生物传感器技术领域，公开了一种基于G4核酸适配体的检测生物分子的方法。所述方法包括如下步骤：(1)在反应溶剂I中，将生物分子的G4核酸适配体与钾离子进行混合反应，得到反应溶液I；(2)将所述反应溶液I、氯化血红素和含有所述生物分子的待测物进行混合I，得到反应溶液II；(3)将所述反应溶液II、过氧化氢和3,3',5,5'-四甲基联苯胺进行混合，得到反应溶液III