Construction method of high-activity traditional Chinese medicine feed additive trypsin

• Main Authors: XIA SHENG, LIU YANHUI, LIANG DAMING, TIAN MEIHUA, QI YIQING, WANG YUWAN, HU YAN, WANG WEI, JIANG HUI, YOU XIHUO, ZHANG YAO, LIU JIALI, ZENG XUHAO, YOU WANGDAN, REN YANAN, SUN HE, XUE DONGSHENG, YU JINGJING, SUN ZHE, SHEN LI
• Format: Patent
• Language: Chinese; English
• Published: 18.03.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a construction method of a high-activity traditional Chinese medicine feed additive trypsin, and belongs to the technical field of genetic engineering. According to the construction method of the high-activity traditional Chinese medicine feed additive trypsin disclosed by the invention, the trypsin with higher activity at 37 DEG C is obtained through a gene mutation technology (error-prone PCR), and the specific enzyme activity is 2436 (BAEE unit)/mg. The enzyme has a wide application prospect in the field of feeds, and has important significance in the breeding industry. 本发明公开了一种高活性中药饲料添加剂胰蛋白酶的构建方法，属于基因工程技术领域。本发明公开的一种高活性中药饲料添加剂胰蛋白酶的构建方法，通过基因突变技术(易错PCR)，得到一个在37℃具有更高活性的胰蛋白酶，比酶活为2436(BAEE单位)/mg。该酶在饲料领域具有广阔的应用前景，对养殖行业具有重要的意义。