High performance liquid chromatography method for determining components in halometasone cream

• Main Authors: NIE LIYUN, LIU YUNFENG, LI HAIDAO, ZHAO HUAJIAN, WEI FURONG
• Format: Patent
• Language: Chinese; English
• Published: 15.03.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a high performance liquid chromatography analysis method for determining components in halometasone cream, which comprises the following steps: (1) preparation of a test solution: taking a proper amount of sample, precisely weighing, dissolving with tetrahydrofuran, washing with a saturated saline solution, collecting an organic phase, volatilizing to dryness to obtain a white solid, precisely weighing the solid, and preparing the test solution; dissolving with an initial proportion mobile phase and fixing the volume to a scale to obtain a test solution; (2) setting a reference solution; (3) chromatographic condition and sample introduction procedure: a chromatographic column adopted by the method is a C18 reversed-phase chromatographic column, ammonium formate buffer salt is taken as a mobile phase A, acetonitrile is taken as a mobile phase B, and gradient elution is carried out, the method provides reliable impurity spectrum reference for research on related substances of the halometa