Establishment method and application of nibea albiflora swim bladder cell line

• Main Authors: LIN FAN, YUAN YUYING, GUO HAOJI, ZHANG HAORAN, LIN JIALI, WANG SHUQI, YU JUN
• Format: Patent
• Language: Chinese; English
• Published: 15.03.2022
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention relates to the technical field of biology, in particular to an establishment method of a nibea albiflora swim bladder cell line and application of the nibea albiflora swim bladder cell line in collagen synthesizing.The establishment method comprises the following steps that S1, the nibea albiflora swim bladder cell line is established through primary culture and subculture, and at present, 50 generations of the nibea albiflora swim bladder cell line are subcultured; and S2, incubating the cells by using a TGF-beta reagent, collecting the cells after 48 hours, and exploring the change of collagen related gene expression quantity and collagen content. Establishment of the nibea albiflora swim bladder cell line as an in-vitro model for researching a collagen synthesis mechanism and swim bladder nutrition regulation is very necessary, the blank in related fields is filled, and a new thought is provided for developing a method for improving the quality of swim bladders. 本发明涉及生物技术领域，具体涉及一种浅色黄姑鱼鱼鳔细胞系的建