Construction method of cellulase high-expression strain suitable for fermentation of traditional Chinese medicine feed

• Main Authors: XIA SHENG, LIU YANHUI, LIANG DAMING, TIAN MEIHUA, QI YIQING, WANG YUWAN, HU YAN, WANG WEI, JIANG HUI, YOU XIHUO, ZHANG YAO, LIU JIALI, ZENG XUHAO, YOU WANGDAN, REN YANAN, SUN HE, XUE DONGSHENG, YU JINGJING, SUN ZHE, SHEN LI
• Format: Patent
• Language: Chinese; English
• Published: 11.03.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FODDER; FOODS OR FOODSTUFFS; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

The invention discloses a construction method of a cellulase high-expression strain adaptive to traditional Chinese medicine feed fermentation, and belongs to the technical field of genetic engineering. The invention discloses a construction method of a cellulase high-expression strain adaptive to traditional Chinese medicine feed fermentation. The construction method comprises the following steps: synthesizing a gene segment, namely GTCGAC-vesicle fusion protein coding sequence-AAGCTT; the preparation method comprises the following steps: respectively carrying out double enzyme digestion, connection, transformation and plasmid extraction on a synthesized gene segment and a pCAMBIA1300 plasmid, so as to obtain pCAMBIA1300-B; agrobacterium tumefaciens-mediated pCAMBIA1300-B is used for transforming aspergillus niger, and an aspergillus niger strain 136 is obtained through screening. The filter paper enzyme activity of fermentation liquor of the aspergillus niger strain 136 is 2.6 U/mL, the enzyme activity of c