Monoclonal antibody protection liquid and preparation method thereof

• Main Authors: CHU YANLIANG, MENG RUIQI, CHU FUMIN
• Format: Patent
• Language: Chinese; English
• Published: 11.03.2022
• Subjects: CHEMISTRY; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES

The invention provides a monoclonal antibody protection liquid and a preparation method thereof, and relates to the technical field of biological material preparation. According to the monoclonal antibody protection liquid and the preparation method thereof, the monoclonal antibody protection liquid is composed of an RPMI-1640 culture medium stock solution, an L.G. Solution, a 7.5% sodium bicarbonate solution, an HEPES solution, ultrapure water and a sterilization solution. Comprising the following steps: S1, preparing and uniformly mixing an RPMI-1640 culture medium stock solution, an L.G. Solution and a 7.5% sodium bicarbonate solution, and S2, weighing 1 mol/L of an HEPES solution, taking ultrapure water, and dissolving the HEPES solution in the pure water. When a common solution is used for degerming, the solution in the previous step is affected by bacteria or degerming is incomplete, so that silver ion sterilization is increased, sterilization timeliness is guaranteed, bacteria generated after productio