Schizochytrium limacinum FR-908 capable of efficiently accumulating DHA and beta-carotene as well as preparation method and application of schizochytrium limacinum FR-908

• Main Authors: TAN WENJUN, CAI YUFENG, LIANG LING, CHEN JIAN, HUANG QINGENG, HUANG WEIQIAN, WENG XUEQING, LIN WANHUA, ZHANG WEN, WANG ZHENZHEN
• Format: Patent
• Language: Chinese; English
• Published: 04.03.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to schizochytrium limacinum FR-908 capable of efficiently accumulating DHA (docosahexaenoic acid) and beta-carotene as well as a preparation method and application thereof. The schizochytrium limacinum FR-908 is preserved in the China Center for Type Culture Collection (CCTCC) on September 15, 2021, and the preservation number of the schizochytrium limacinum FR-908 is CCTCC (China Center for Type Culture Collection) NO: M20211181. Schizochytrium limacinum and rhodosporidium torulosporidium starting strains are respectively used for preparing protoplasts, the protoplasts are subjected to biparental inactivation in a heating mode, polyethylene glycol is used as a fusion promoting agent for cell fusion and regeneration, and the high-yield and high-fat-content high-protein protein powder with high fermentation biomass and high fat content is obtained through flat plate preliminary screening and shake flask fermentation screening. Meanwhile, the novel fused schizochytrium limacinum can be use