Fipronil rapid quantitative detection kit and detection method

• Main Authors: HUANG LIPING, LIU GANG, ZENG SHAOQI, PENG QINGZHI, GONG LEI, ZHOU TAOHONG, FENG HAO, JI WEIHAO, PI JIANGYI, LI SHIYAO
• Format: Patent
• Language: Chinese; English
• Published: 01.03.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention belongs to the technical field of detection, and particularly discloses a fipronil rapid quantitative detection kit and a detection method.The fipronil rapid quantitative detection kit comprises a nano plasma resonance sensing detection plate and a detection reagent; the detection plate is prepared by arranging a nano plasma resonance sensing chip in holes of a bottomless hole plate, and each sample hole of the detection plate is coated with a fipronil antigen; the detection reagent comprises fipronil standard mother liquor, a fipronil gold-labeled antibody, a detection buffer solution and a fipronil extraction and purification reagent. During detection, a fipronil standard solution with known concentration and a pretreated sample solution to be detected are respectively added into the sample holes; adding a gold-labeled antibody for reaction; and drawing a standard curve according to the OD change value of the specific wavelength before and after the gold-labeled antibody is added, so as to rea