Method for enhancing demethylmercuric methyl and/or divalent mercury reduction capacity of fungus, recombinant fungus and application

• Main Authors: BAO YUTING, WU CONGCONG, FANG WEIGUO
• Format: Patent
• Language: Chinese; English
• Published: 01.03.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DISPOSAL OF SOLID WASTE; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECLAMATION OF CONTAMINATED SOIL; RECLAMATION OF CONTAMINED SOIL SOIL; SPIRITS; TRANSPORTING; VINEGAR; WINE

The invention provides a method for enhancing demethylmercuric methyl and/or divalent mercury reduction capacity of fungi, a recombinant strain and application, and relates to the technical field of recombinant strains. By utilizing the method disclosed by the invention, a recombinant fungus for independently and excessively expressing a demethylated mercury methylase gene Mmd or a divalent mercury reductase Mir from metarhizium robertianum and a recombinant engineering fungus for simultaneously expressing Mmd and Mir genes can be obtained. Compared with wild fungi, the recombinant fungi and the recombinant engineering fungi have the advantage that the methyl mercury methyl removal and/or divalent mercury reduction capability can be obviously enhanced. 本发明提供了一种增强真菌去甲基汞甲基和/或二价汞还原能力的方法、重组菌株和应用，涉及重组菌株技术领域。利用本发明所述方法，可获得分别单独超量表达来自罗伯茨绿僵菌的去甲基汞甲基酶基因Mmd或二价汞还原酶Mir的重组真菌，和同时表达Mmd和Mir基因的重组工程真菌。本发明所述重组真菌和重组工程真菌相比于野生菌，可显著增强其去甲基汞甲基和/或二价汞还原能力。