Amino acid sequence of anti-okadaic acid single-chain antibody and expression vector thereof

• Main Authors: TIAN YINQI, XIE ZENGHONG, LIN XUCONG, YUAN LIN
• Format: Patent
• Language: Chinese; English
• Published: 01.03.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention particularly relates to an amino acid sequence of an anti-okadaic acid single-chain antibody and an expression vector of the anti-okadaic acid single-chain antibody. The amino acid sequence comprises an amino acid sequence of a heavy chain variable region as shown in SEQ ID No.1; and an amino acid sequence of a light chain variable region, which is shown as SEQ ID No.2. A nucleotide sequence for coding the heavy chain variable region is as shown in SEQ ID No.3, and a nucleotide sequence for coding the light chain variable region is as shown in SEQ ID No.4. A soluble recombinant plasmid is formed by a nucleotide sequence of the single-chain antibody and a pET28a-MBP-T7-6 * His expression vector of maltose binding protein with a histidine tag. 本发明具体涉及了一条抗大田软海绵酸的单链抗体的氨基酸序列及其表达载体。所述氨基酸序列包括：一重链可变区的氨基酸序列，其如SEQ ID No.1所示；一轻链可变区的氨基酸序列，其如SEQ ID No.2所示。编码该重链可变区的核苷酸序列如SEQ ID No.3所示，编码该轻链可变区的核苷酸序列如SEQ ID No.4所示。所述单链抗体的核苷酸序列与带有组氨酸标签麦芽糖结合蛋白的pET28a-MBP-T7-6×His表达载体形成了可溶性重组质粒。