Pseudovirus particle capable of being used for evaluating activity of new coronavirus neutralizing antibody and serving as nucleic acid detection standard substance and preparation method of pseudovirus particle

• Main Authors: ZHAO WEN, DOU XIANGYA, YANG HUI, WANG XINYUE, SHAO DONGYAN, TIAN YIXIAO, BAI YUEQIU, HAN CUICUI, LI YUHANG, HUANG QINGSHENG, DUAN SUYANG
• Format: Patent
• Language: Chinese; English
• Published: 22.02.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention provides a pseudovirus particle which can be used for evaluating the activity of a new coronavirus neutralizing antibody and used as a nucleic acid detection standard substance and a preparation method of the pseudovirus particle. The problems of biological potential safety hazards caused by the adoption of new crown live viruses for neutralizing antibody effect evaluation in current new crown antiviral research and potential safety hazards such as virus replication possibly existing in preparation of a pseudovirus nucleic acid standard substance for new crown nucleic acid detection are solved. The pseudovirus particle takes lentivirus as a framework; the outer membrane protein is the outer membrane protein fused by a VSV-G protein and a new coronavirus S protein receptor binding motif; the core gene of the gene is an SARS-CoV-2 virus gene segment; the SARS-CoV-2 virus gene segment is a concatemer of seven RNA (Ribonucleic Acid) segments of the virus; the seven RNA fragments are respectively a b