Fluorescent probe for detecting ferroheme as well as preparation method and application of fluorescent probe

• Main Authors: WANG HENG, LIU SHENG, ZHANG CHONGJING, YU SHISHAN, WEI CHUNYAN
• Format: Patent
• Language: Chinese; English
• Published: 22.02.2022
• Subjects: ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAININGELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN,SULFUR, SELENIUM OR TELLURIUM; ADHESIVES; CHEMISTRY; DYES; HETEROCYCLIC COMPOUNDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE; MEASURING; METALLURGY; MISCELLANEOUS APPLICATIONS OF MATERIALS; MISCELLANEOUS COMPOSITIONS; NATURAL RESINS; ORGANIC CHEMISTRY; PAINTS; PHYSICS; POLISHES; TESTING

The invention belongs to the technical field of analytical chemistry, and relates to a fluorescent probe for detecting ferroheme as well as a preparation method and application thereof, in particular to a fluorescent probe for detecting heme and plasmodium as well as a preparation method and application thereof. The fluorescent probe is a Light-up fluorescent probebased on Yingzhaosu A, and the design of the fluorescent probe is based on a mechanism that the decomposition of the fluorescent probe is catalyzed by heme which is generated by hydrolyzing hemoglobin by plasmodium in host red blood cells so as to cause the leaving of a fluorescence resonance energy transfer effect group, so the detection of plasmodium such as plasmodium falciparum is realized, and the advantages of high analysis sensitivity, high selectivity and the like are obtained. According to the invention, plasmodium can be detected by the aid of flow cytometry, tedious staining processes of the traditional Giemsa staining method can be omitt