High-efficiency expression method of aspergillus niger glucose oxidase gene

• Main Authors: MA XIAOTAO, SHI YUPEI, ZHANG CHANJUAN, ZHANG JUNFENG, YAN LINGPENG, REN YANGQI, HU HONGWEI, DANG ZHUO, CHEN BOSHENG, YANG DAMOU
• Format: Patent
• Language: Chinese; English
• Published: 18.02.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the field of gene engineering, in particular to a coding gene of glucose oxidase and a gene engineering expression strain thereof. The invention provides an efficient pichia pastoris expression method of an aspergillus niger glucose oxidase gene. In order to improve the correct folding of recombinase, the protein disulfide bond isomerase pdi gene is introduced into the engineering bacteria through tandem carrier construction, so that the protein folding is helped, and the enzyme expression activity is improved. The invention also provides a fermentation medium composition and a process for producing glucose oxidase from pichia pastoris. 本发明涉及基因工程领域，具体地，本发明涉及一种葡萄糖氧化酶的编码基因及其基因工程表达菌株。本发明提供了一种黑曲霉葡萄糖氧化酶基因的高效巴斯德毕赤酵母表达方法。为了提高重组酶的正确折叠，在工程菌中通过串联载体构建，引入了蛋白质二硫键异构酶pdi基因，帮助蛋白质折叠，提高酶的表达活性。本发明还提供了一种毕赤酵母生产葡萄糖氧化酶的发酵培养基组成及工艺。