Chondrosulphatase B as well as coding gene and construction method thereof

The invention provides chondrosulphatase B, a coding gene and a construction method thereof, and the chondrosulphatase B has amino acid as shown in SEQ ID NO: 1. The chondrosulphatase B can effectively carry out enzymolysis on a chondroitin sulfate B substrate into disaccharide, the optimum pH is 8....

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Main Authors MOU MINGJING, DU XIPING, LI ZHIPENG, ZHENG MINGJING, JIANG ZEDONG, LI LIJUN, ZHU YANBING, NI HUI, YANG YUANFAN, HUANG TIANXIANG
Format Patent
LanguageChinese
English
Published 15.02.2022
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
SPIRITS
VINEGAR
WINE
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Summary:The invention provides chondrosulphatase B, a coding gene and a construction method thereof, and the chondrosulphatase B has amino acid as shown in SEQ ID NO: 1. The chondrosulphatase B can effectively carry out enzymolysis on a chondroitin sulfate B substrate into disaccharide, the optimum pH is 8.0, and the optimum temperature is 40 DEG C; the heat stability is good, and the residual activity is about 50% after treatment at 60 DEG C for 2 h; the recombinase has good stability in a pH range of 4.0 to 10.0. The recombinase shows excellent stability to a non-ionic surface active agent (Triton X-100, Tween 20 and Tween 80) to be tested and a cationic surface active agent CTAB (Cetyltrimethyl Ammonium Bromide). Through enzymolysis of the chondroitin sulfate B, the oxidation resistance of the chondroitin sulfate B serving as the hydroxyl free radical scavenger is improved. The activity and stability of the enzyme make it competitive candidates for further fundamental research, medicine, industrial and biotechnolo
Bibliography:Application Number: CN202111205435