Method for detecting cell cancerization degree by using circular RNA circKIF4A and miR-1231 sequence adsorption quantity

• Main Authors: WANG NINGMEI, MU HAIDONG, ZHANG WENCAN
• Format: Patent
• Language: Chinese; English
• Published: 11.02.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

According to the present invention, a circular RNA circKIF4A sequence and an miR-1231 specific sequence produce the adsorption effect, the high-throughput sequencing is adopted to identify the targeting of the circular RNA circKIF4A to the miR-1231 specific sequence, the adsorption effect results in the canceration process of the prostate cancer cells, and the higher the m6A methylation modification percentage of the circKIF4A specific sequence is, the stronger the expression inhibition of the miR-1231 on the prostate cancer cell ferroptosis key gene is;. 环状RNA circKIF4A序列与miR-1231特定序列产生吸附作用，通过高通量测序鉴定环状RNA circKIF4A靶向miR-1231特定序列，吸附作用导致前列腺癌细胞癌变的进程，所述的circKIF4A特定序列的m6A甲基化修饰百分比越高，则导致miR-1231对前列腺癌细胞铁死亡关键基因的表达抑制越强。