Primer and probe for detecting madeira mealybug based on fluorescent quantitative PCR (Polymerase Chain Reaction), kit and method

• Main Authors: GUO HUILIN, FANG WENYUAN, TIAN HONGWEI, WU ZHIYI, HUANG FANG, TANG HUIJI, LIU PENGJUAN, CHENG FAN, DANG ZHIHAO, XU MIAOFENG, ZHANG YENA, REN YAN
• Format: Patent
• Language: Chinese; English
• Published: 08.02.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a primer and a probe for detecting madeira mealybug based on fluorescent quantitative PCR (polymerase chain reaction) as well as a kit and a detection method adopting the primer and the probe. The detection primer group comprises an upstream primer MD-F, a downstream primer MD-R and a probe MD-Probe; and the detection kit comprises a primer, a probe, a template, a positive sample, a negative sample and a premixed solution. The detection method comprises the following steps: extracting DNA of a to-be-detected sample, quickly detecting the DNA of the to-be-detected sample by using a fluorescent quantitative PCR technology, and judging whether the to-be-detected sample is madeira mealybug or not according to the fluorescent quantitative PCR amplification condition. The method has the advantages of rapidness, sensitivity, specificity, easiness in operation and the like, and is convenient to popularize and apply. 本发明公开了一种基于荧光定量PCR检测美地绵粉蚧的引物和探针，以及采用了该引物和探针的试剂盒和检测方法。检测引物组包括上游引物MD-F、下游引物MD-R和探