Construction method of mitochondrial genome sequencing library based on high-throughput sequencing and amplification primer

• Main Authors: XIAO RUI, LEI JIHAI, HUA DASONG
• Format: Patent
• Language: Chinese; English
• Published: 08.02.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMBINATORIAL CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a construction method of a mitochondrial genome sequencing library based on high-throughput sequencing and an amplification primer. The amplification primer comprises a primer pair 1 and a primer pair 2; and the nucleotide sequences of the primer pair 1 are shown as SEQ ID NO.1 and SEQ ID NO.2, and the nucleotide sequences of the primer pair 2 are shown as SEQ ID NO.3 and SEQ ID NO.4. According to the invention, only two pairs of amplification primers are needed, and a sufficient quantity of mitochondrial DNA samples can be obtained through one-time PCR amplification, so that the complex primer quantity and the cost input are greatly reduced, and the operation is simplified. According to the invention, the amplification products of the two pairs of primers are mixed to construct the library, so that the integrity and the accuracy of the mtDNA are improved, and the effectiveness of the data analysis is ensured. 本发明公开了一种基于高通量测序的线粒体基因组测序文库的构建方法及扩增引物。该扩增引物包括引物对1和引物对2；引物对1的核苷酸序列如SEQ ID NO.1