Cellulase for promoting release of traditional Chinese medicine feed additive in intestinal tract and production method

• Main Authors: XIA SHENG, LIU YANHUI, LIANG DAMING, TIAN MEIHUA, QI YIQING, WANG YUWAN, HU YAN, WANG WEI, JIANG HUI, YOU XIHUO, ZHANG YAO, LIU JIALI, ZENG XUHAO, YOU WANGDAN, REN YANAN, SUN HE, XUE DONGSHENG, YU JINGJING, SUN ZHE, SHEN LI
• Format: Patent
• Language: Chinese; English
• Published: 08.02.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses cellulase for promoting release of a traditional Chinese medicine feed additive in intestinal tracts. The amino acid sequence of the cellulase is shown as SEQ ID NO.1, and the sequence for coding the cellulase is shown as SEQ ID NO.2; the production method of the cellulase comprises the following steps: carrying out PCR (Polymerase Chain Reaction) by taking a synthesized sequence Endo1 as a template to obtain a target gene; carrying out BamHI and EcoRI double enzyme digestion on a target gene and a pRSFduet-1 plasmid, and then connecting an enzyme digestion product to obtain a recombinant plasmid pET28a-endo; transforming the recombinant plasmid pET28a-endo into an escherichia coli competent cell, and obtaining a transformed strain; and carrying out induced culture on the transformed strain, centrifuging, taking a supernatant, and purifying to obtain the cellulase. 本发明公开了一种促进中药饲料添加剂在肠道释放的纤维素酶，纤维素酶的氨基酸序列如SEQ ID NO.1所示，编码该酶的序列如SEQ ID NO.2所示；纤维素酶的生产方法，包括：以合成的序列Endo1为模板，进行PCR，获得目的基因；对目的基因和