Preparation method of light mineral oil for embryo culture

• Main Authors: WU YUANYUAN, YAN ZHENGJIE, SUN MIAO, YUE TING, HOU DAORONG, LIU YINYAN, CHEN XUEMEI
• Format: Patent
• Language: Chinese; English
• Published: 08.02.2022
• Subjects: CHEMISTRY; CRACKING HYDROCARBON OILS; FUELS; LUBRICANTS; METALLURGY; MINERAL WAXES; PEAT; PETROLEUM, GAS OR COKE INDUSTRIES; PRODUCTION OF LIQUID HYDROCARBON MIXTURES, e.g. BY DESTRUCTIVEHYDROGENATION, OLIGOMERISATION, POLYMERISATION; RECOVERY OF HYDROCARBON OILS FROM OIL-SHALE, OIL-SAND, ORGASES; REFINING MIXTURES MAINLY CONSISTING OF HYDROCARBONS; REFORMING OF NAPHTHA; TECHNICAL GASES CONTAINING CARBON MONOXIDE

The invention discloses a preparation method of light mineral oil for embryo culture. Light mineral oil (with density of 0.84-0.86) as a raw material, ethylenediamine tetraacetic acid as a main purifying agent and sterilized water for injection as a solvent are purified by an ethanol solution, a sodium chloride solution and an ethylenediamine tetraacetic acid solution to obtain light mineral oil for embryo culture. The preparation method is simple, convenient and rapid, and the content of heavy metal, endotoxin and nucleic acid in the mineral oil can be effectively reduced. When the light mineral oil obtained through the method is used for embryo culture, a high blastocyst forming rate can be obtained. The light mineral oil is suitable for embryo culture operation of universities, colleges, scientific research institutions and reproductive centers. 本发明公开了一种胚胎培养用轻质矿物油的制备方法，以轻质矿物油（密度0.84-0.86）为原料，主要纯化剂为乙二胺四乙酸，溶剂为灭菌注射用水，经乙醇溶液、氯化钠溶液、乙二胺四乙酸溶液纯化后得到胚胎培养用轻质矿物油，本制备方法简便快速，可有效降低矿物油内重金属、内毒素、核酸的含量，用本方法获得的轻质矿物油进行胚胎培养，可获得较高