Experimental method for treating POI by combining ASP with hAD-MSCs

• Main Authors: ZHANG YUNHAN, MO XIAOLI, HE LIANLI, XIE HONGMEI, ZHANG QINGYUN, ZHU YE, LONG XIAOQIN
• Format: Patent
• Language: Chinese; English
• Published: 08.02.2022
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

The invention relates to an experimental method for treating POI by combining ASP with hAD-MSCs. The experimental method comprises the following steps: preparing ASP drug-containing serum; adding ASP drug-containing serum with different concentrations into a culture medium to culture hAD-MSCs; and carrying out hAD-MSCs transplantation; 5) carrying out experiment grouping; observing ultrastructure change of each level of follicles and endometrium by using an electron microscope; carrying out immunohistochemical determination on the expression of a granular cell surface marker Fshr and an oocyte surface marker DDX4; and using an Elisa method for determining secretion conditions of antioxidant enzymes and oxidation products ROS, SOD and the like of the ovary and uterus tissues; (13) carrying out Western-blot determining and obtaining the change condition of the phosphorylation level; determining apoptosis conditions of follicular granular cells and oocytes by Tunel staining; determining the expression of the ova