Chromatographic method for simultaneously analyzing liraglutide and Boc-liraglutide main chain thereof

• Main Authors: CHEN WEI, ZHANG ZHENSHAN, TU JIANFEI, WU SONG
• Format: Patent
• Language: Chinese; English
• Published: 28.01.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a chromatographic method for simultaneously analyzing liraglutide and Boc-liraglutide main chains thereof. The method specifically comprises the following steps: injecting a sample solution containing liraglutide and Boc-liraglutide main chains into a high performance liquid chromatograph, and performingdetecting by adopting the following high performance liquid chromatography conditions: bonding a silica gel chromatographic column, wherein the mobile phase A is a 0.05 mol/mL-0. 15 mol/mL ammonium dihydrogen phosphate aqueous solution, and the mobile phase B is acetonitrile, and the volume ratio of the mobile phase B is 15%->95%, and gradient elution is carried out. The analysis method has the advantages of being high in analysis speed, accurate, simple, convenient and rapid, capable of playing a good role in quality control, analysis and purification in the liraglutide production process, good in specificity, high in sensitivity, low in cost and the like. 本发明公开了一种同时分析利拉鲁肽及其Boc-利拉鲁肽主链的