Method for producing protocatechuic acid

• Main Authors: WANG ZHIJIAN, JIA WEIMIN, CHENG CUNZHAO, NIU SHANPO, CHEN MINGZHAO
• Format: Patent
• Language: Chinese; English
• Published: 28.01.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the field of fermentation of biological spice intermediates, and particularly relates to a method for producing protocatechuic acid, which comprises the step of adding 3-dehydroshikimic acid dehydratase into fermentation liquor in the process of producing 3-dehydroshikimic acid through fermentation to obtain protocatechuic acid. By adopting the method disclosed by the invention, the yield of gallic acid can be greatly reduced, and even gallic acid cannot be produced; in addition, after fermentation is finished, fermentation liquor (which can reach 80g/L) containing high-concentration protocatechuic acid can be obtained, and the concentration of the fermentation liquor is far higher than that of the fermentation liquor when protocatechuic acid producing bacteria are directly used for fermentation production of protocatechuic acid. 本发明属于生物香料中间体发酵领域，具体涉及生产原儿茶酸的方法，该方法包括：在发酵生产3-脱氢莽草酸的过程中，向发酵液中添加3-脱氢莽草酸脱水酶，得到原儿茶酸。采用本发明的方法能够大大降低没食子酸的产量，甚至能够不产没食子酸；而且发酵结束后能够获得含高浓度原儿茶酸的发酵液(能达80g/L)，远高于直接使用原儿茶酸生