Novel coronavirus neutralizing antibody as well as preparation method and application thereof

• Main Authors: FANG SHUBIN, LIN JIZHEN
• Format: Patent
• Language: Chinese; English
• Published: 21.01.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention provides a high-affinity neutralizing antibody of a spike protein receptor binding domain (S-RBD) for specific targeting SARS-CoV-2, the inhibition ratio IC50 of the neutralizing antibody to SARS-CoV-2 pseudovirus is 1.597 [mu]g/ml, and the affinity KD value of the neutralizing antibody is 10 <-10 > M level. The neutralizing antibody can block the mutual reaction between the S-RBD and the human ACE2 receptor, such that the virus cannot enter the human epithelial cell, the ability of the SARS-CoV-2 virus to infect the host is inhibited, and the neutralizing antibody can be used for treating the infectious diseases caused by the SARS-CoV-2 coronavirus or other similar coronaviruses. The invention also provides nucleic acid molecules for encoding the antibodies of the invention, expression vectors, host cells and methods for expressing the antibodies of the invention, and pharmaceutical compositions comprising the antibodies of the invention. 本发明提供了一种特异性靶向SARS-CoV-2的刺突蛋白(spike蛋白)受体结合域(S-RBD)的高亲和力中和