Specific primer pair for detecting anti-ALS inhibitor herbicide brome, kit and detection method and application

• Main Authors: WANG JIANPING, XU XIAN, WANG GUIQI, SHEN BEIBEI, LIU XIAOMIN, LI BINGHUA, QI ZHIZUN, CUI HAIYAN, ZHAO BOCHUI
• Format: Patent
• Language: Chinese; English
• Published: 18.01.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a specific primer pair for detecting anti-ALS inhibitor herbicide brome, a kit, a detection method and application, and relates to the technical field of molecular biology. A specific strip is good in specificity, and a PCR target strip is clear. By utilizing the specific primer pair or the kit containing the specific primer pair, anti-ALS inhibitor herbicide bromegrass can be accurately and specifically identified, and the detection method is simple and convenient to operate, high in speed, low in cost and high in accuracy. The invention further provides a detection method based on the specific primer pair and the kit, ALS mutation sites and amino acid mutation types of field suspected resistant brome can be efficiently and accurately detected, and whether ALS inhibitor herbicides can be continuously used for preventing and removing resistant brome or not can be guided. 本发明提供了检测抗ALS抑制剂类除草剂雀麦的特异性引物对、试剂盒及检测方法和应用，涉及分子生物学技术领域。本发明所述特异性条带特异性好，PCR目的条带清晰。利用本发明所述特异性引物对或包含所述特异性引物对的试剂盒，可准确专一的鉴定出抗