基于液相色谱-质谱检测血清中特瑞普利单抗的方法

• Format: Patent
• Language: Chinese
• Published: 14.11.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

本发明提供一种基于液相色谱-质谱检测血清中特瑞普利单抗的方法，属于单克隆抗体检测技术领域，其利用商品化的稳定同位素标记的通用单克隆抗体作为内标，并采用琼脂糖基质磁性微球实现人血清样品中的特瑞普利单抗的富集，酶解获得特征性肽段，通过内标法定量特征性肽段从而准确定量人血清中特瑞普利单抗的含量，具有特异性高、高通量、灵敏度高、抗干扰能力强的优点。 The invention provides a method for detecting a tereprenil monoclonal antibody in serum based on liquid chromatography-mass spectrometry and belongs to the technical field of monoclonal antibody detection. The method comprises steps that a commercialized stable isotope labeled universal monoclonal antibody is used as an internal standard, and agarose matrix magnetic microspheres are adopted to achieve enrichment of the tereprenil monoclonal antibody in a human serum sample; a specific peptide fragment is quantified through an internal standard method so as to accurately quantify the content of the specific peptide fragment in the human serum, and the specific peptide fragment has advantages of high specificity, high flux, high sensitivity and strong anti-interference capability.