KASP molecular marker and method for detecting high-temperature-resistant gene TT1 of rice

• Main Authors: CAO LIMING, TU RONGJIAN, CHENG CAN, NIU FU'AN, CHU HUANGWEI, ZHOU JIHUA, SUN BIN
• Format: Patent
• Language: Chinese; English
• Published: 14.01.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a KASP molecular marker and method for detecting a high-temperature-resistant gene TT1 of rice. The molecular marker is designed according to difference of basic groups of a second SNP locus of the TT1 gene and is named as TT1-KASP. Primers of the KASP marker comprise two forward specific primers and one reverse universal primer. Genetic typing is carried out according to the KASP marker provided by the invention, enzyme digestion and electrophoresis are not needed after PCR amplification, a plurality of samples can be detected at high throughput, the distribution condition of TT1 alleles in rice segregation populations can be rapidly detected, the result accuracy is good, and compared with a conventional molecular marker, the KASP marker has the advantages of rapidness, simplicity, convenience, lower cost and the like. The KASP molecular marker has an important significance in molecular-marker-assisting selective breeding of the high-temperature-resistant gene TT1 of the rice. 本发明公开了一