Sheep kobuvirus VP1 recombinant protein, ELISA detection kit and preparation method and application thereof

• Main Authors: TANG CHENG, WANG YUANWEI, YUE HUA, ABIKE HAMO
• Format: Patent
• Language: Chinese; English
• Published: 14.01.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses sheep kobuvirus VP1 recombinant protein, an ELISA detection kit as well as a preparation method and application of the sheep kobuvirus VP1 recombinant protein. The amino acid sequence of the sheep kobuvirus VP1 recombinant protein is as shown in SEQ ID NO. 1. The preparation method of the sheep-derived kobuvirus VP1 recombinant protein comprises the following steps: extracting sheep-derived kobuvirus nucleic acid, amplifying, optimizing a gene sequence to obtain an optimized nucleotide sequence, constructing a recombinant prokaryotic expression vector, inducing expression and purifying to obtain the sheep-derived kobuvirus VP1 recombinant protein. The invention also provides an ELISA detection kit, which mainly comprises an ELISA plate coated with the sheep kobuvirus VP1 recombinant protein. The sheep kobuvirus VP1 recombinant protein disclosed by the invention is high in specificity, easy to purify and prepare and low in cost, and can be used as an antigen to simply, quickly and accur