Method for co-producing trehalose and gluconic acid

• Main Authors: CUI HUIHUI, JIANG XINYI, CHEN YIMING, GUO JING, MAN ZAIWEI
• Format: Patent
• Language: Chinese; English
• Published: 11.01.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for co-producing trehalose and gluconic acid. A safe strain corynebacterium glutamicum is used for overexpressing trehalose synthase, triton X-100 is used for treating and recombining corynebacterium glutamicum to obtain permeable cells, and whole-cell catalysis maltose can be used for synthesizing trehalose. Aspergillus niger AnM1 thalli capable of producing gluconic acid are added into reaction liquid for catalytically preparing trehalose, glucose in a system is oxidized into gluconic acid, the inhibition effect of glucose on trehalose synthase activity can be reduced, and the trehalose synthesis rate is increased. After the trehalose synthesis reaction is balanced, the reaction liquid is centrifuged, corynebacterium glutamicum and aspergillus niger thalli are collected, and the obtained thalli can be reused. Saccharifying enzyme and aspergillus niger AnM1 thalli are added into the supernatant of the reaction liquid, the residual maltose in the reaction liquid is hydrolyzed