Alfalfa embryonic development late protein MsLEA1 and coding gene and application thereof

• Main Authors: XU RUI, AN YUAN, GAO LI, FAN NANA, LYU AIMIN
• Format: Patent
• Language: Chinese; English
• Published: 04.01.2022
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses alfalfa embryonic development late protein MsLEA1 and a coding gene and application thereof, and relates to the field of plant genetic engineering. An expression vector containing the coding gene of the protein MsLEA1 is constructed, a plant host is converted, and a transgenic plant is obtained through cultivation and screening. The gene expression of the alfalfa 'WL525' embryonic development late protein MsLEA1 is regulated and controlled through the genetic engineering technology, and therefore the aluminum resistance and drought resistance of alfalfa are improved, an important theoretical basis is provided for cultivation work of a new alfalfa variety, and great application value is achieved. 本发明公开了一种紫花苜蓿胚胎发育晚期蛋白MsLEA1及其编码基因和应用，涉及植物基因工程领域，通过构建含有蛋白MsLEA1的编码基因的表达载体，并转化植物宿体，培育筛选得到转基因植株，本发明利用基因工程技术调控紫花苜蓿'WL525'胚胎发育晚期蛋白MsLEA1基因表达，从而提高了紫花苜蓿耐铝、抗旱性，为紫花苜蓿新品种的培育工作提供了重要的理论依据，具有很大的应用价值。