Quantitative determination method of vaccinia virus lister strain based on ddPCR

• Main Authors: LIN FANJIA, HAN JIADI, ZHANG YAN, SUN JING, CHENG XIN, SHAN YIWEI
• Format: Patent
• Language: Chinese; English
• Published: 31.12.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a quantitative determination method of a vaccinia virus lister strain based on ddPCR, and belongs to the technical field of PCR detection. The invention provides a method for quantitatively determining the vaccinia virus lister strain in a sample. The method comprises the following steps of: firstly extracting virus DNA in the sample, then taking a specific fragment as a probe, taking the extracted virus DNA as a template, and performing quantitative analysis on the vaccinia virus lister strain in the sample by using the ddPCR. The method is based on the ddPCR, the ddPCR can perform absolute quantification on nucleic acid molecules and is high in sensitivity, and meanwhile, the ddPCR does not need a standard substance, is less in variables, higher in accuracy and lower in detection lower limit, and is more suitable for quantitative determination of the vaccinia virus lister strain with a low copy number, so that the method for quantitatively determining the vaccinia virus lister strai