Gene for coding recombinant avian influenza virus HA protein, virus-like particle, vaccine, preparation and application

• Main Authors: FAN HUIYING, CHEN TAORAN, HU XIAOLONG, LIAO MING, KONG DEXIN
• Format: Patent
• Language: Chinese; English
• Published: 31.12.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of genetic engineering vaccines, and particularly relates to a gene for coding recombinant avian influenza virus HA protein, a virus-like particle, a vaccine, preparation and application. The gene has a nucleotide sequence as shown in SEQ ID NO: 1. The invention also provides an avian influenza virus-like particle, the avian influenza virus-like particle is assembled by HA protein, NA protein and M1 protein, and has a hemagglutination titer up to 13log2. The invention also provides an avian influenza virus-like particle vaccine containing the virus-like particle, the vaccine can provide complete clinical protection and significantly inhibit detoxification against lethal attack of homologous and wild H7N9 subtype highly pathogenic avian influenza viruses, and a new vaccine choice is provided for prevention and control of H7N9 subtype avian influenza. 本发明属于基因工程疫苗技术领域，具体涉及一种编码重组禽流感病毒HA蛋白的基因、病毒样颗粒、疫苗及制备与应用。所述的编码重组禽流感病毒HA蛋白的基因的核苷酸序列如SEQ ID NO:1。本发明还提供了一种禽流感病毒样颗粒，该禽流感病毒样