MAD7-NLS fusion protein, nucleic acid construct for site-directed editing of plant genome and application of nucleic acid construct

• Main Authors: LI YINGYING, LIU ZHENG, LIU ZIJIA, PEI RUILI, ZHOU HONGJU, LI XIANGGAN, ZHENG HUAYING
• Format: Patent
• Language: Chinese; English
• Published: 28.12.2021
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention provides an MAD7-NLS fusion protein, which has the following structure: B1-C-B2, B1-C or C-B2, wherein C is MAD7 protein; and b1 and B2 are independently nuclear localization signal (NLS) sequences. The invention also provides a nucleic acid construct for site-directed editing of a pla...