MAD7-NLS fusion protein, nucleic acid construct for site-directed editing of plant genome and application of nucleic acid construct

• Main Authors: LI YINGYING, LIU ZHENG, LIU ZIJIA, PEI RUILI, ZHOU HONGJU, LI XIANGGAN, ZHENG HUAYING
• Format: Patent
• Language: Chinese; English
• Published: 28.12.2021
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention provides an MAD7-NLS fusion protein, which has the following structure: B1-C-B2, B1-C or C-B2, wherein C is MAD7 protein; and b1 and B2 are independently nuclear localization signal (NLS) sequences. The invention also provides a nucleic acid construct for site-directed editing of a plant genome,which comprises a first expression cassette, and the first expression cassette comprises a first promoter, a coding nucleotide sequence of the MAD7-NLS fusion protein and a first terminator which are connected in sequence. The invention also provides application of the nucleic acid construct in plant gene editing. The MAD7 can be used for simply, conveniently and efficiently carrying out single-gene knockout, multi-gene knockout or homologous recombination and directional insertion of exogenous fragments at predetermined plant genome sites. 本发明提供一种MAD7-NLS融合蛋白，具有以下结构：B1-C-B2、B1-C或C-B2，其中，C为MAD7蛋白；B1和B2各自独立地为核定位信号序列(NLS)。本发明还提供了用于植物基因组定点编辑的核酸构建物，包含第一表达盒，第一表达盒包含依次连接的第一启动子、MAD7-NLS融合蛋白的编码核苷酸序列和第一终止子。本发明还提供了该