Preparation method and application of nanogel with hunger combined gas therapy anti-tumor function

• Main Authors: CHEN WEI, DAI LIN, HUANG DECHUN, NIU YAFAN
• Format: Patent
• Language: Chinese; English
• Published: 21.12.2021
• Subjects: CHEMISTRY; COMPOSITIONS BASED THEREON; HUMAN NECESSITIES; HYGIENE; MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC MACROMOLECULAR COMPOUNDS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; THEIR PREPARATION OR CHEMICAL WORKING-UP

The invention discloses a preparation method and application of nanogel with a hunger combined gas therapy anti-tumor function. The nanogel is prepared by the following steps: firstly, performing electrostatic interaction on polyethyleneimine azide grafted with L-arginine and glucose oxidase to form a nano-enzyme compound; and then covering the surface of the nano-enzyme compound with an alkynyl-containing polycarbonate block copolymer, and performing click chemical crosslinking on azide and alkynyl. Compared with the prior art, according to the method disclosed by the invention, specific enzymatic reaction is utilized to consume endogenous glucose and release nitric oxide gas, cancer cells are killed through hunger and gas therapy, besides, the nitric oxide gas can redistribute oxygen in the cancer cells, hypoxia of tumors is relieved, and wide application prospects are realized in the aspects of resisting tumors and inhibiting tumor metastasis. 本发明公开了一种具有饥饿联合气体疗法抗肿瘤功能的纳米凝胶的制备方法和应用，所述纳米凝胶先由接枝左旋精氨酸的叠氮化聚乙烯亚胺与葡