SELF-CALIBRATING AND DIRECTIONAL FOCUSING SYSTEMS AND METHODS FOR INFINITY CORRECTED MICROSCOPES

• Main Author: CHAN MATTHEW
• Format: Patent
• Language: Chinese; English
• Published: 14.12.2021
• Subjects: OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS; OPTICS; PHYSICS

Systems and methods for autofocusing an objective lens in a microscope system. A self-calibrating autofocus system includes a light source, a decentered aperture, and image-capturing device. The system may be connected to the microscope system so that the light source generates a light on an optical path that includes the objective lens and a plate having a reference surface proximal to a sample holding component. The image capturing device images a reflection of light from the reference surface as the objective lens moves to a series of z-positions. A reference calibration slope is generated for the objective lens by determining positions of the images taken at the z-positions of the objective lens. At least one image having a particular attribute corresponds to a best focus position. The objective lens is moved to predicted best focus or preferred offset from calibrated best focus using the reference calibration slope. 用于显微镜系统中的物镜的自动聚焦的系统和方法。自校准自动聚焦系统包括光源、偏心孔和图像捕获设备。该系统可以连接到显微镜系统，使得光源在包括物镜和板的光路上产生光，该板具有在样品保