Construction method of glucose biosensor assembled based on glutathione

• Main Authors: LIU NING, JI XIA, GAO XIANJUAN, DU QINGQING, REN YUNFENG
• Format: Patent
• Language: Chinese; English
• Published: 07.12.2021
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention belongs to the technical field of biosensors, and particularly relates to a construction method of a glucose biosensor assembled based on glutathione. The method comprises the following steps: by taking a glutathione modified gold electrode as a carrier, activating carboxylated graphene, loading the activated carboxylated graphene on the surface of the glutathione modified gold electrode to obtain a carboxylated graphene/glutathione modified gold electrode, soaking the carboxylated graphene/glutathione modified gold electrode in a cross-linking agent, and airing to obtain the glucose biosensor assembled based on glutathione. According to the invention, the glutathione-carboxylated graphene self-assembled glucose biosensor is successfully assembled. Glutathione and carboxylated graphene are used for immobilizing glucose oxidase, and the glucose biosensor with high sensitivity, good selectivity, good reproducibility and good stability is constructed. 本发明属于生物传感器技术领域，具体涉及一种基于谷胱甘肽组装的葡萄糖生物传感器的构建方法。该方法