Vector and method for expressing L-aspartic acid-alpha-decarboxylase by recombinant Escherichia coli

• Main Authors: JIANG TAILONG, QIU GUISEN, LIU WENJIE
• Format: Patent
• Language: Chinese; English
• Published: 03.12.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a vector for expressing L-aspartic acid-alpha-decarboxylase in recombinant Escherichia coli, and a method for expressing the L-aspartic acid-alpha-decarboxylase by using the corresponding vector. According to the method, the expression quantity of the target protein can be ensured while the formation of inclusion body products is reduced. The method is suitable for industrial large-scale production and reduces the cost. 本发明提供一种在重组大肠杆菌表达L-天冬氨酸-α-脱羧酶的载体，以及使用相应载体的表达L-天冬氨酸-α-脱羧酶的方法。所述方法能够在减少包涵体产物形成的同时，保证目标蛋白的表达量。适用于工业化大规模生产，降低成本。