Induced reinforcement preparation process and application of poultry mesenchymal stem cell exosomes

• Main Authors: XIE RUSHENG, JIN YUTANG
• Format: Patent
• Language: Chinese; English
• Published: 26.11.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses an induced reinforcement preparation process of poultry mesenchymal stem cell exosomes, which comprises the following steps: performing primary isolation culture on poultry mesenchymal stem cells, performing subculture, taking the poultry mesenchymal stem cells, performing culture by using a cell growth solution, discarding the culture solution, firstly washing the cells for three times by using a phosphate buffer solution, adding curcumin to stimulate and continuously culturing for 72 hours by using a serum-free culture solution, collecting the serum-free culture solution, and continuously carrying out the treatment on the uncollected cells until enough supernatant is collected; and centrifuging the collected curcumin-stimulated serum-free culture solution, extracting and concentrating an exosome filtrate, and carrying out degerming treatment on the obtained exosome concentrated filtrate. According to the preparation process, the technological formula is improved, the poultry mesenchy