Artificial inoculation method for plant bacterial vascular bundle diseases

• Main Authors: ZHONG LIHUA, OUYANG QIUFEI, LI XI, WEI LANJIE
• Format: Patent
• Language: Chinese; English
• Published: 19.11.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an artificial inoculation method for plant bacterial vascular bundle diseases, which comprises the following steps: step 1) preparation of a bacterial solution: inoculating pathogenic bacteria on an NA culture medium, conducting culturing for 24 hours at 30 DEG C, then conducting transferring once again, washing off bacterial lawn on a flat plate by using a sterilized 0.2-0.3 mol/L PB buffer solution, and preparing a bacterial suspension with the thallus concentration of 0.5*10 -1.5*10 cfu/mL; 2) treatment of in-vitro branches: cutting new branches with 4-5 unfolded leaves at the tops from the woody plants, putting the new branches into a 75% ethanol solution for disinfection for 1-3 minutes, and washing the new branches with sterilized water for multiple times; and 3) inoculating: inserting the branches into a triangular flask or a glass cup filled with 1/3-1/2 volume of the bacterial suspension, wrapping the branches with cotton or sponge, conducting sealing and fixing, and conductin