Method for detecting related substances of 6-chloro-1,3-indolin-2-one

• Main Author: CHEN NIANGEN
• Format: Patent
• Language: Chinese; English
• Published: 12.11.2021
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a method for detecting related substances of 6-chloro-1,3-indolin-2-one. The adopted chromatographic conditions are as follows: the chromatographic column is InertsilODS-3 (4.6*250 mm, 5 [mu] m), the column temperature is 25-35 DEG C, the detection wavelength is 210-220 nm, the mobile phase adopts a phosphate buffer liquid (the pH is adjusted to be 2.9-3.1 by phosphoric acid) as a phase A, the mobile phase adopts acetonitrile as a phase B, and the flow velocity is 0.9-1.1 ml/min. According to the method disclosed by the invention, the related substances in the 6-chloro-1,3-indolin-2-one can be accurately and quantitatively analyzed, the 6-chloro-1,3-indolin-2-one can be effectively separated from impurities, the specificity, the precision and the durability all meet the requirements, and the detection effect is good. 本发明提供一种用于检测6-氯-1,3-二氢吲哚-2-酮有关物质的方法，采用的色谱条件为：色谱柱为InertsilODS-3(4.6×250mm，5μm)，柱温为25～35℃，检测波长210～220nm，流动相为以磷酸盐缓冲液体(磷酸调节pH＝2.9～3.1)为A相，以乙腈为B相，流速为0.9～1.1ml/min。利用本发明的方法可以准确地进行