Preparation method and application of human papilloma virus HPV16 type E7 active protein

• Main Authors: WANG YING, XU YA, YIN TINGZI, YI WANGXUE, ZHANG ZHEN, CAI YING, YU QIAN, KE HONG
• Format: Patent
• Language: Chinese; English
• Published: 12.11.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses a preparation method and application of a human papilloma virus HPV16 type E7 active protein. The known coding gene of the HPV16 type E7 protein is subjected to codon optimization to obtain an HPV16 type E7 protein coding gene as shown in SEQ ID NO.1, and an Escherichia coli fermentation system is used for in-vitro expression, so that the solubility of a target protein is remarkably improved, the HPV16 type E7 protein is expressed as a soluble protein, the situation that the target protein is expressed in an inclusion body form and needs to be subjected to denaturation, dissolution and renaturation again to obtain the active protein is avoided, so that the preparation efficiency of the E7 protein is remarkably improved, and the HPV16 type E7 protein with high purity and natural activity can be efficiently obtained. The method is simple to operate and low in cost, and the HPV16 type E7 active protein prepared by the method can be used as an antigen for preparing an anti-HPV therapeutic