Mycobacterium tuberculosis multi-line drug resistance gene identification method and device

• Main Authors: XIE ZHIHAO, YUAN JIANYING, LI YINGZHEN, MAO WANSI
• Format: Patent
• Language: Chinese; English
• Published: 09.11.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a mycobacterium tuberculosis multi-line drug resistance gene identification method and device. The method comprises the following steps: acquiring a whole genome sequence obtained by metagenome sequencing of a sample or a sequence of a mycobacterium tuberculosis drug-resistant target region obtained on the basis of targeted amplification sequencing, and carrying out data quality control; comparing the sequence subjected to the data quality control to a mycobacterium tuberculosis genome, sequencing and screening out a comparison result meeting the quality requirement; carrying out local assembly and variation detection on the sequences of the gene regions where the variation sites of which the comparison positions are located in the mycobacterium tuberculosis drug resistance gene database are located; annotating a variation detection result to the mycobacterium tuberculosis drug resistance gene database according to the positions and mutation types of variation sites on the genome; and