Detection kit for determining gene SNP related to GLP-1 receptor stimulant curative effect

• Main Authors: YANG ZHIYAN, LI YUE, DU YUE, GUAN ZIWAN, YANG YILEI, LI YAN, CUI XUEYAN, WANG XINYU
• Format: Patent
• Language: Chinese; English
• Published: 05.11.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a detection kit for determining gene SNP related to a GLP-1 receptor stimulant curative effect, belongs to the technical field of genes, and judges the curative effect expectation of the treatment of the GLP-1 receptor stimulant (GLP1RAs) by measuring sequencing of the single nucleotide polymorphism (SNP) of a plurality of sites of a GLP1R gene so as to guide and adjust a treatment scheme of a patient with type 2 diabetes. Specifically, the SNP sites are specifically rs3765467, rs10305420, rs6923761 and rs761386, a forward primer and a reverse primer of each site are designed, and a collected sample is subjected to PCR amplification, purification, re-amplification and sequencing. The kit provides a theoretical basis for clinical individualized medication, and also provides a guidance basis for new drug research and development on the basis of a pharmacogenomics concept. 本发明提供一种用于确定GLP-1受体激动剂疗效相关的基因SNP检测试剂盒，属于基因技术领域，通过测定对GLP1R基因的多个位点的单核苷酸多态性（SNP）进行测序，从而判断对于GLP-1受体激动剂（GLP1RAs）治疗的疗效预期，从而指导