Recombinant miR-218 as well as production method and application thereof in hair regeneration

• Main Authors: QIAN JING, QIAN AIRONG, TAN SHENXING, CHEN ZHIHAO, TIAN YE, HAN JIANGFAN
• Format: Patent
• Language: Chinese; English
• Published: 05.11.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses a recombinant miR-218. The sequence of the recombinant miR-218 is as shown in SEQ ID NO: 1, or is a sequence of which the similarity with the sequence as shown in SEQ ID NO: 1 is 90% or above. In addition, the invention also discloses a production method of the recombinant miR-218 and an application of the recombinant miR-218 in hair regeneration. According to the method, tRNA is adopted as a scaffold, an miR-218 sequence is embedded, and recombinant miR-218 is expressed in escherichia coli. The recombinant miR-218 prepared by the invention is externally sprayed to the back of depilated C57BL/6 mice. The result proves that the recombinant miR-218 can obviously promote hair regeneration. The recombinant miR-218 prepared by the invention is obtained by a bioengineering technology, and has the advantages of high biological activity, convenient equipment, fast production, high yield, low cost, good functionality and the like. 本发明公开了一种重组miR-218，所述重组miR-218的序列如SEQ ID NO：1所示，或者为与SEQ ID NO：1所示