METHOD AND KIT FOR MEASURING APP669-711

A method for measuring APP669-711 that comprises: an immobilization step for immobilizing a first antibody, which is capable of immunospecifically binding to the N-terminus of APP669-711, on a support; a blocking step for blocking the support, on which the first antibody is immobilized, with a block...

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Bibliographic Details
Main Author KANEKO NAOKI
Format Patent
LanguageChinese
English
Published 02.11.2021
Subjects
CHEMISTRY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PHYSICS
TESTING
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Summary:A method for measuring APP669-711 that comprises: an immobilization step for immobilizing a first antibody, which is capable of immunospecifically binding to the N-terminus of APP669-711, on a support; a blocking step for blocking the support, on which the first antibody is immobilized, with a blocking agent; a first reaction step for contacting APP669-711 in a sample with the immobilized first antibody and thus allowing APP669-711 to bind to the first antibody; a sample-removal step for removing the unbound sample; a second reaction step for contacting a second antibody, which is capable of immunospecifically binding to the C-terminus of APP669-711, with APP669-711 bound to the immobilized first antibody and thus allowing the second antibody to bind to APP669-711; a second antibody-removal step for removing the unbound second antibody; and a detection step for detecting the presence of the second antibody bound to APP669-711. 一种APP669-711测定方法，包括：固定化工序，将能与APP669-711的N末端免疫特异性地结合的第1抗体固定化于支撑体；封闭工序，将固定化有上述第1抗体的支撑
Bibliography:Application Number: CN202080017569