Sample AFAI-DESI detection method and application thereof

• Main Authors: YANG XIANFA, LU JIAWEI, HU ZHE, FU YANLEI, JI PEIPEI, SHU LIEBO
• Format: Patent
• Language: Chinese; English
• Published: 02.11.2021
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a sample AFAI-DESI detection method, which comprises the following steps: dyeing a sample obtained after a tissue sample is frozen and sliced, and detecting by AFAI-DESI, the horizontal displacement rate is 0.03-0.05 mm/s, the vertical displacement distance is 0.01-0.03 mm, the atomization gas flow is 0.65-0.75 MPa, the spray solvent flow rate is 5.5-6.5 [mu]L/min, the extending length of a spray needle is 0.45-0.55 mm, and the included angle between a DESI spray needle and a glass slide is 45-55 degrees. The invention further provides application of the method to improvement of spatial resolution. According to the sample AFAI-DESI detection method and the application thereof provided by the invention, the spatial resolution of the technology is improved to 20 microns, and meanwhile, the metabolite detection sensitivity and coverage are ensured. 本发明提供一种样本AFAI-DESI检测方法，包括：将组织样本冰冻切片后获得的样片染色，采用AFAI-DESI进行检测，水平位移速率为0.03-0.05mm/s，垂直位移距离为0.01-0.03mm，雾化气流量为0.65-0.75MPa，喷雾溶剂流速为5.5-6.5μL/min，喷针伸