Mutagenesis method of new Pyrus Betulaefolia Bunge variant variety on the basis of tissue culture system
The invention discloses a mutagenesis method of a new Pyrus Betulaefolia Bunge variant variety on the basis of a tissue culture system. The mutagenesis method comprises the following steps of: before a tree sprouts in spring, collecting a one-year-old lignified branch on a mutagenesis method seed tr...
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Main Authors | , , , , , , , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
02.11.2021
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Subjects | |
Online Access | Get full text |
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Summary: | The invention discloses a mutagenesis method of a new Pyrus Betulaefolia Bunge variant variety on the basis of a tissue culture system. The mutagenesis method comprises the following steps of: before a tree sprouts in spring, collecting a one-year-old lignified branch on a mutagenesis method seed tree; cutting the branch into a scion, and keeping a notch flat; carrying out oblique cutting for one time on a position which is 1cm away from the back of the lower bud of the scion to form a long cut surface; obliquely cutting a small cut surface on the back of the long cut surface; keeping three complete and full buds on the scion; truncating a trunk at the position, which is 5cm away from the ground, of a stock, flattening a section, and vertically cutting the position, which is provided with slight xylem, in cortex by 3cm; preparing a sodium azide solution, soaking the scion in the sodium azide solution, taking out the scion, and inserting the scion into the notch of the stock; and wrapping the joint of the scio |
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Bibliography: | Application Number: CN202110930946 |