Mutagenesis method of new Pyrus Betulaefolia Bunge variant variety on the basis of tissue culture system

• Main Authors: LU PING, XIE XIAN, LI CHENGXIU, QIAO QIAN, YANG BO, SUN ZHONGKUI, REN HONGJIAN, ZHANG ANQI, CHENG TIANTIAN, ZHANG LIN, YU YONGCHANG, YAN YU
• Format: Patent
• Language: Chinese; English
• Published: 02.11.2021
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; CHEMISTRY; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED; FERTILISERS; FERTILISERS CHARACTERISED BY THEIR FORM; FISHING; FORESTRY; HORTICULTURE; HUMAN NECESSITIES; HUNTING; MANUFACTURE THEREOF; METALLURGY; MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENTSUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVINGA SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES,SOIL-CONDITIONERS, WETTING AGENTS; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING; WATERING

The invention discloses a mutagenesis method of a new Pyrus Betulaefolia Bunge variant variety on the basis of a tissue culture system. The mutagenesis method comprises the following steps of: before a tree sprouts in spring, collecting a one-year-old lignified branch on a mutagenesis method seed tree; cutting the branch into a scion, and keeping a notch flat; carrying out oblique cutting for one time on a position which is 1cm away from the back of the lower bud of the scion to form a long cut surface; obliquely cutting a small cut surface on the back of the long cut surface; keeping three complete and full buds on the scion; truncating a trunk at the position, which is 5cm away from the ground, of a stock, flattening a section, and vertically cutting the position, which is provided with slight xylem, in cortex by 3cm; preparing a sodium azide solution, soaking the scion in the sodium azide solution, taking out the scion, and inserting the scion into the notch of the stock; and wrapping the joint of the scio