Primer combination for detecting human bocavirus whole genome and detection method thereof

• Main Authors: ZHU ZHAOKUI, SHEN LIN
• Format: Patent
• Language: Chinese; English
• Published: 26.10.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of virus detection, in particular to a primer combination for detecting a human bocavirus whole genome and a detection method thereof. The invention provides the primer combination for detecting the human bocavirus whole genome, the primer combination comprises 12 pairs of primers, and the 12 pairs of primers have nucleotide sequences as shown in SEQ ID NO.1-24. The primer combination can cover more than 98% of the the human bocavirus whole genome sequence, has human bocavirus specificity, and has universality for different human bocaviruses; and the primer combination can realize multiple-PCR one-step amplification of the human bocavirus whole genome in a PCR reaction system, is high in sensitivity, can quickly construct a bocavirus genome library, and is used for high-throughput sequencing. 本发明涉及病毒检测技术领域，具体涉及一种检测人博卡病毒全基因组的引物组合及其检测方法。本发明提供一种用于人博卡病毒全基因组检测的引物组合，其包括12对引物，所述12对引物具有如SEQ ID NO.1-24所示的核苷酸序列。上述引物组合能够覆盖人博卡病毒的全基因组序列约98％以上区域，具有人博卡病毒特异性，且对于不同的人博卡病毒具有通用性；上述引物组